2017-2018年我国部分地区牛支原体的分离鉴定及多位点序列分型

doi:10.11843/j.issn.0366-6964.2019.09.013

Abstract

Abstract: The purpose of this study is to isolate Mycoplasma bovine (M. bovis) epdemic strains in some areas of China and analyse the genetic characteristics and evolutionary relationship of M. bovis. Three hundred and twenty-seven samples were collected from cattle with respiratory symptoms in several large-scale farms of Heilongjiang, Hebei, Jiangsu, Anhui and Inner Mongolia between 2017 and 2018. Twenty strains of M. bovis were cultured and identified by 16S rRNA sequencing. The isolates were classified according to Multiple Locus Sequence Typing (MLST) with the adoption of 7 housekeeping genes including adh1,gltX,gpsA,gyrB,pta2,tdk and tkt. In order to analyze the genetic characteristics and evolutionary relationship of M. bovis isolates, the minimum spanning tree of 20 clinical isolates and 85 strains of M. bovis published in GenBank database were set up. The results showed that MycbPAN005 was identified as ST-6 and the other strains were identified as ST-10, indicating that ST-10 was the main epidemic type in China. All M. bovis in China belong to CC1 category and there is only one housekeeping gene different among ST-32, ST-26, ST-43 and ST-10 except for ST-6, which indicate that the population structure of M. bovis is relatively steady in China.This is the first report domestically about ST-6 strain, providing new materials for epidemic disease database research of M. bovis.

CLC Number:

S852.62

KANG Haoran, LIU Chongyang, YU Yong, ZHANG Junjie, PAN Zihao, YAO Huochun. Isolation,Identification and Multilocus Sequence Typing Analysis of Mycoplasma bovis Strains in Some Regions of China during 2017 to 2018[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1857-1863.

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Isolation,Identification and Multilocus Sequence Typing Analysis of Mycoplasma bovis Strains in Some Regions of China during 2017 to 2018

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